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	Provedor de dados AgEcon (1) BJM (1) Autor Cabrera-Crespo,Joaquim (1) Ferreira,Luís Carlos Souza (1) Lasaro,Marcio de Oliveira (1) Lasaro,Melissa Ang-Simões (1) Rodrigues,Juliana Falcão (1) Sbrogio-Almeida,Maria Elisabete (1) Mais... Palavra-chave LT (2) CELISA (1) Cage (1) Coccidiosis (1) Competitive exclusion (1) Contract farm (1) ETEC (1) Egg (1) Flock (1) Heat-labile toxin (1) Infectious bronchitis (1) Laryngotracheitis (1) Lay (1) Layer (1) Layers (1) Livestock Production/Industries (1) Molt (1) Mycoplasma gallisep (1) NAHMS (1) Newcastle disease (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Report (1) Ano 2007 (1) 1999 (1) País Brazil (1) United States (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Evaluation of experimental conditions for quantification of LT produced by human derived enterotoxigenic Escherichia coli strains BJM Lasaro,Melissa Ang-Simões; Rodrigues,Juliana Falcão; Cabrera-Crespo,Joaquim; Sbrogio-Almeida,Maria Elisabete; Lasaro,Marcio de Oliveira; Ferreira,Luís Carlos Souza. The heat-labile toxin (LT) is a key virulence-associated factor associated with the non-invasive secretory diarrhea caused by enterotoxigenic Escherichia coli (ETEC) strains either in humans or domestic animals. Several LT detection methods have been reported but quantification of the toxin produced by wild-type ETEC strains is usually performed by the GM1 ganglyoside enzyme-linked immunosorbent assay (GM1 ELISA). In this study we conducted the optimization of an alternative LT-quantification method, the antibody-capture ELISA (cELISA). Detailed analysis of the appropriate dilutions of capture and detecting LT-specific antibodies significantly improved the sensitivity of the method. Additionally, testing of different LT extraction techniques indicated that... Tipo: Info:eu-repo/semantics/article Palavras-chave: Heat-labile toxin; LT; ETEC; CELISA. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300012 LAYERS '99, PART I: REFERENCE OF 1999 TABLE EGG LAYER MANAGEMENT IN THE U.S. AgEcon The National Animal Health Monitoring System (NAHMS) is sponsored by the USDA:APHIS:Veterinary Services (VS). The NAHMS Layers '99 Study was designed to provide information about the nation's layer population for education and research purposes. The USDA's National Agricultural Statistics Service (NASS) collaborated with VS to select a statistically-valid sample from 15 states for Layers '99. The 15-state target population accounted for over three-quarters of the table egg layers in the U.S. as of December 1, 1998. The study was limited to companies with 30,000 or more layers. While the majority of farm sites were contract farms (61.0 percent), the majority of birds were located on company owned farms (72.6 percent), indicating that company owned farms... Tipo: Report Palavras-chave: NAHMS; Layer; Layers; Poultry; Pullet; Flock; Egg; Table egg; Lay; Molt; Cage; Contract farm; Coccidiosis; Newcastle disease; Infectious bronchitis; Salmonella enteritidis; S.e.; Competitive exclusion; Laryngotracheitis; LT; Mycoplasma gallisep; Livestock Production/Industries. Ano: 1999 URL: http://purl.umn.edu/32757 Registros recuperados: 2 Primeira ... 1 ... Última

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